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Effects of non-muscle myosin ⅡA silenced bone marrow mesenchymal stem cells on lung damage of rats at early stage of smoke inhalation injury.

Abstract Objective: To investigate the effects of non-muscle myosin ⅡA (NMⅡA) silenced bone marrow mesenchymal stem cells (BMSCs) on the lung damage of rats at early stage of smoke inhalation injury. Methods: Forty Sprague-Dawley rats were divided into control, simple injury, NMⅡA-BMSCs, and BMSCs groups according to the completely random method, with 10 rats in each group. Rats in control group inhaled air normally, while rats in the latter 3 groups inhaled smoke to reproduce model of smoke inhalation injury. At 30 min post injury, rats in simple injury group were injected with 1 mL normal saline via caudal vein, and rats in group BMSCs were injected with 1 mL the fifth passage of BMSCs (1×10(7)/mL), and rats in group NMⅡA-BMSCs were injected with 1 mL NMⅡA silenced BMSCs (1×10(7)/mL). At post injury hour (PIH) 24, abdominal aorta blood and right lung of rats in each group were harvested, and then arterial partial pressure of oxygen (PaO(2)), arterial partial pressure of carbon dioxide (PaCO(2)), and pH value were detected by blood gas analyzer. Ratio of wet to dry weight of lung was determined by dry-wet weight method. Pathological changes of lung were observed with HE staining. Bronchoalveolar lavage fluid (BALF) were collected, and then tumor necrotic factor-α (TNF-α) and interleukin-10 (IL-10) content of BALF was determined by enzyme-linked immunosorbent assay. Data were processed with one-way analysis of variance, Kruskal-Wallis H test, and least-significant difference test. Results: (1) At PIH 24, compared with those in control group, PaO(2) values of rats in simple injury, BMSCs, and NMⅡA-BMSCs groups were obviously decreased (with P values below 0.05), and PaCO(2) values were obviously increased (with P values below 0.05). Compared with those in simple injury group, PaO(2) values of rats in groups NMⅡA-BMSCs and BMSCs were obviously increased (with P values below 0.05), while PaCO(2) values were obviously decreased (with P values below 0.05). PaO(2) value of rats in group NMⅡA-BMSCs was obviously increased as compared with that in group BMSCs (P<0.05). The pH value of arterial blood of rats in simple injury group was obviously lower than that in control group (P<0.05). (2) At PIH 24, ratios of wet to dry weight of lung of rats in control, simple injury, BMSCs, and NMⅡA-BMSCs groups were 4.36±0.15, 7.79±0.42, 5.77±0.18, and 5.11±0.20, respectively. Compared with that in control group, ratio of wet to dry weight of lung of rats was obviously increased in the other 3 groups (with P values below 0.05). Compared with that in simple injury group, ratio of wet to dry weight of lung of rats was obviously decreased in groups BMSCs and NMⅡA-BMSCs (with P values below 0.05). Compared with that in group BMSCs, ratio of wet to dry weight of lung of rats in group NMⅡA-BMSCs was obviously decreased (P<0.05). (3) At PIH 24, alveolar structure of rats in control group was complete without abnormality. Compared with those in simple injury group, lung injury and infiltration of inflammatory cells of rats in groups BMSCs and NMⅡA-BMSCs were obviously alleviated, and alveolar structure was relatively complete with no thickening of alveolar wall. (4) At PIH 24, compared with that in control group, TNF-α content of BALF of rats in simple injury and BMSCs groups was obviously increased (with P values below 0.05). Compared with that in simple injury group, TNF-α content of BALF in groups BMSCs and NMⅡA-BMSCs was obviously decreased (with P values below 0.05). Compared with that in control group, IL-10 content of BALF in simple injury, NMⅡA-BMSCs and BMSCs groups were obviously increased (with P values below 0.05). Compared with that in simple injury group, IL-10 content of BALF in groups BMSCs and NMⅡA-BMSCs was obviously increased (with P values below 0.05). Compared with that in group BMSCs, IL-10 content of BALF in group NMⅡA-BMSCs was obviously increased (P<0.05). Conclusions: NMⅡA silenced BMSCs can alleviate lung damage of rats at early stage of smoke inhalation injury, showing better effectiveness than using BMSCs only.
PMID
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Authors

Mayor MeshTerms
Keywords

Inflammatory response

Inhalation injury

Lung injury

Mesenchymal stem cells

Nonmuscle myosin type ⅡA

Journal Title zhonghua shao shang za zhi = zhonghua shaoshang zazhi = chinese journal of burns
Publication Year Start




PMID- 29275618
OWN - NLM
STAT- In-Process
LR  - 20171225
IS  - 1009-2587 (Print)
IS  - 1009-2587 (Linking)
VI  - 33
IP  - 12
DP  - 2017 Dec 20
TI  - [Effects of non-muscle myosin A silenced bone marrow mesenchymal stem cells on
      lung damage of rats at early stage of smoke inhalation injury].
PG  - 766-771
LID - 10.3760/cma.j.issn.1009-2587.2017.12.009 [doi]
AB  - Objective: To investigate the effects of non-muscle myosin A (NMA) silenced bone 
      marrow mesenchymal stem cells (BMSCs) on the lung damage of rats at early stage
      of smoke inhalation injury. Methods: Forty Sprague-Dawley rats were divided into 
      control, simple injury, NMA-BMSCs, and BMSCs groups according to the completely
      random method, with 10 rats in each group. Rats in control group inhaled air
      normally, while rats in the latter 3 groups inhaled smoke to reproduce model of
      smoke inhalation injury. At 30 min post injury, rats in simple injury group were 
      injected with 1 mL normal saline via caudal vein, and rats in group BMSCs were
      injected with 1 mL the fifth passage of BMSCs (1x10(7)/mL), and rats in group
      NMA-BMSCs were injected with 1 mL NMA silenced BMSCs (1x10(7)/mL). At post injury
      hour (PIH) 24, abdominal aorta blood and right lung of rats in each group were
      harvested, and then arterial partial pressure of oxygen (PaO(2)), arterial
      partial pressure of carbon dioxide (PaCO(2)), and pH value were detected by blood
      gas analyzer. Ratio of wet to dry weight of lung was determined by dry-wet weight
      method. Pathological changes of lung were observed with HE staining.
      Bronchoalveolar lavage fluid (BALF) were collected, and then tumor necrotic
      factor-alpha (TNF-alpha) and interleukin-10 (IL-10) content of BALF was
      determined by enzyme-linked immunosorbent assay. Data were processed with one-way
      analysis of variance, Kruskal-Wallis H test, and least-significant difference
      test. Results: (1) At PIH 24, compared with those in control group, PaO(2) values
      of rats in simple injury, BMSCs, and NMA-BMSCs groups were obviously decreased
      (with P values below 0.05), and PaCO(2) values were obviously increased (with P
      values below 0.05). Compared with those in simple injury group, PaO(2) values of 
      rats in groups NMA-BMSCs and BMSCs were obviously increased (with P values below 
      0.05), while PaCO(2) values were obviously decreased (with P values below 0.05). 
      PaO(2) value of rats in group NMA-BMSCs was obviously increased as compared with 
      that in group BMSCs (P&lt;0.05). The pH value of arterial blood of rats in simple
      injury group was obviously lower than that in control group (P&lt;0.05). (2) At PIH 
      24, ratios of wet to dry weight of lung of rats in control, simple injury, BMSCs,
      and NMA-BMSCs groups were 4.36+/-0.15, 7.79+/-0.42, 5.77+/-0.18, and 5.11+/-0.20,
      respectively. Compared with that in control group, ratio of wet to dry weight of 
      lung of rats was obviously increased in the other 3 groups (with P values below
      0.05). Compared with that in simple injury group, ratio of wet to dry weight of
      lung of rats was obviously decreased in groups BMSCs and NMA-BMSCs (with P values
      below 0.05). Compared with that in group BMSCs, ratio of wet to dry weight of
      lung of rats in group NMA-BMSCs was obviously decreased (P&lt;0.05). (3) At PIH 24, 
      alveolar structure of rats in control group was complete without abnormality.
      Compared with those in simple injury group, lung injury and infiltration of
      inflammatory cells of rats in groups BMSCs and NMA-BMSCs were obviously
      alleviated, and alveolar structure was relatively complete with no thickening of 
      alveolar wall. (4) At PIH 24, compared with that in control group, TNF-alpha
      content of BALF of rats in simple injury and BMSCs groups was obviously increased
      (with P values below 0.05). Compared with that in simple injury group, TNF-alpha 
      content of BALF in groups BMSCs and NMA-BMSCs was obviously decreased (with P
      values below 0.05). Compared with that in control group, IL-10 content of BALF in
      simple injury, NMA-BMSCs and BMSCs groups were obviously increased (with P values
      below 0.05). Compared with that in simple injury group, IL-10 content of BALF in 
      groups BMSCs and NMA-BMSCs was obviously increased (with P values below 0.05).
      Compared with that in group BMSCs, IL-10 content of BALF in group NMA-BMSCs was
      obviously increased (P&lt;0.05). Conclusions: NMA silenced BMSCs can alleviate lung 
      damage of rats at early stage of smoke inhalation injury, showing better
      effectiveness than using BMSCs only.
FAU - Liu, M Z
AU  - Liu MZ
AD  - Department of Burns, the First Affiliated Hospital of Nanchang University,
      Nanchang 330006, China.
FAU - Wang, J J
AU  - Wang JJ
FAU - Fu, Z H
AU  - Fu ZH
FAU - Li, Y C
AU  - Li YC
FAU - Jiang, Z Y
AU  - Jiang ZY
FAU - Sun, W
AU  - Sun W
FAU - Guo, G H
AU  - Guo GH
FAU - Zhu, F
AU  - Zhu F
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Shao Shang Za Zhi
JT  - Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns
JID - 100959418
OTO - NOTNLM
OT  - Inflammatory response
OT  - Inhalation injury
OT  - Lung injury
OT  - Mesenchymal stem cells
OT  - Nonmuscle myosin type A
EDAT- 2017/12/26 06:00
MHDA- 2017/12/26 06:00
CRDT- 2017/12/26 06:00
PHST- 2017/12/26 06:00 [entrez]
PHST- 2017/12/26 06:00 [pubmed]
PHST- 2017/12/26 06:00 [medline]
PST - ppublish
SO  - Zhonghua Shao Shang Za Zhi. 2017 Dec 20;33(12):766-771.