PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 29277757
OWN - NLM
STAT- MEDLINE
DCOM- 20180101
LR  - 20180104
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 38
IP  - 1
DP  - 2018 Jan
TI  - In Vitro Evaluation of Humanized/De-immunized Anti-PSMA Immunotoxins for the
      Treatment of Prostate Cancer.
PG  - 61-69
AB  - BACKGROUND: We generated humanized/de-immunized immunotoxins targeting the
      prostate-specific membrane antigen (PSMA) and tested their cytotoxic activity
      against prostate cancer cells in vitro. MATERIALS AND METHODS: The
      humanized/de-immunized version of our murine anti-PSMA single-chain antibody
      fragment (scFv) D7, termed hD7-1(VL-VH), was ligated to the 40-kDa toxin domain
      of Pseudomonas aeruginosa exotoxin A (PE40), and to the deimmunized 24-kDa toxin 
      domains PE24 or PE24mut. The immunotoxins designated as hD7-1(VL-VH)-PE40,
      hD7-1(VL-VH)-PE24 and hD7-1(VL-VH)-PE24mut were bacterially expressed and
      purified by affinity chromatography. Binding and cytotoxicity were examined by
      flow cytometry and viability assay, respectively. RESULTS: All immunotoxins
      revealed strong binding to prostate cancer cells expressing PSMA and specific
      cytotoxicity, with half-maximal inhibitory concentration values in the picomolar 
      range. CONCLUSION: We successfully created powerful anti-PSMA immunotoxins with
      reduced immunogenicity for further clinical development and application against
      advanced prostate cancer.
CI  - Copyright(c) 2018, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Michalska, Marta
AU  - Michalska M
AD  - Department of Urology, Medical Center - University of Freiburg, Faculty of
      Medicine, University of Freiburg, Freiburg, Germany.
FAU - Schultze-Seemann, Susanne
AU  - Schultze-Seemann S
AD  - Department of Urology, Medical Center - University of Freiburg, Faculty of
      Medicine, University of Freiburg, Freiburg, Germany.
FAU - Kuckuck, Irina
AU  - Kuckuck I
AD  - Department of Urology, Medical Center - University of Freiburg, Faculty of
      Medicine, University of Freiburg, Freiburg, Germany.
FAU - Wolf, Philipp
AU  - Wolf P
AD  - Department of Urology, Medical Center - University of Freiburg, Faculty of
      Medicine, University of Freiburg, Freiburg, Germany
      [email protected]
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Antigens, Surface)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Exotoxins)
RN  - 0 (Immunotoxins)
RN  - 0 (Virulence Factors)
RN  - EC 2.4.2.- (ADP Ribose Transferases)
RN  - EC 2.4.2.31 (toxA protein, Pseudomonas aeruginosa)
RN  - EC 3.4.17.21 (Glutamate Carboxypeptidase II)
RN  - EC 3.4.17.21 (glutamate carboxypeptidase II, human)
SB  - IM
MH  - ADP Ribose Transferases/genetics/immunology
MH  - Animals
MH  - Antigens, Surface/*immunology
MH  - Bacterial Toxins/genetics/immunology
MH  - Cell Line, Tumor
MH  - Escherichia coli/genetics
MH  - Exotoxins/genetics/immunology
MH  - Glutamate Carboxypeptidase II/*immunology
MH  - Humans
MH  - Immunotoxins/*pharmacology
MH  - Male
MH  - Mice
MH  - Prostatic Neoplasms/drug therapy/*immunology
MH  - Virulence Factors/genetics/immunology
OTO - NOTNLM
OT  - *Immunotoxin
OT  - *PSMA
OT  - *Pseudomonas exotoxin A
OT  - *deimmunization
OT  - *prostate cancer
EDAT- 2017/12/27 06:00
MHDA- 2018/01/02 06:00
CRDT- 2017/12/27 06:00
PHST- 2017/10/11 00:00 [received]
PHST- 2017/10/27 00:00 [revised]
PHST- 2017/11/01 00:00 [accepted]
PHST- 2017/12/27 06:00 [entrez]
PHST- 2017/12/27 06:00 [pubmed]
PHST- 2018/01/02 06:00 [medline]
AID - 38/1/61 [pii]
AID - 10.21873/anticanres.12192 [doi]
PST - ppublish
SO  - Anticancer Res. 2018 Jan;38(1):61-69. doi: 10.21873/anticanres.12192.