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Design, construction and evaluation of multi-epitope antigens for diagnosis of Lyme disease.

Abstract Introduction and objective. Lyme disease (LD) is the most common vector-borne disease in the temperate zone of the Northern Hemisphere. Diagnosis of LD is mainly based on clinical symptoms supported with serology (detection of anti-<i>Borrelia</i> antibodies) and is often misdiagnosed in areas of endemicity.
PMID
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Authors

Mayor MeshTerms
Keywords

ELISA

Lyme disease

Slovakia

chimera

epitope

Journal Title annals of agricultural and environmental medicine : aaem
Publication Year Start




PMID- 29284249
OWN - NLM
STAT- MEDLINE
DCOM- 20180105
LR  - 20180105
IS  - 1898-2263 (Electronic)
IS  - 1232-1966 (Linking)
VI  - 24
IP  - 4
DP  - 2017 Dec 23
TI  - Design, construction and evaluation of multi-epitope antigens for diagnosis of
      Lyme disease.
PG  - 696-701
LID - 80699 [pii]
LID - 10.26444/aaem/80699 [doi]
AB  - INTRODUCTION: Introduction and objective. Lyme disease (LD) is the most common
      vector-borne disease in the temperate zone of the Northern Hemisphere. Diagnosis 
      of LD is mainly based on clinical symptoms supported with serology (detection of 
      anti-&lt;i&gt;Borrelia&lt;/i&gt; antibodies) and is often misdiagnosed in areas of
      endemicity. MATERIAL AND METHODS: In this study, the chimeric proteins (A/C-2,
      A/C-4 and A/C-7.1) consisting of B-cell epitopes of outer surface proteins OspA
      and OspC from &lt;i&gt;Borrelia&lt;/i&gt; genospecies prevalent in Eastern Slovakia, were
      designed, over-expressed in &lt;i&gt;E. coli&lt;/i&gt;, and used to detect specific
      anti-&lt;i&gt;Borrelia&lt;/i&gt; antibodies in serologically characterized sera from patients
      with Lyme-like symptoms to evaluate their diagnostic potential. RESULTS: Results 
      showed that chimeras vary in their immuno-reactivity when tested with human sera.
      Compared with the results obtained from a two-tier test, the application of
      recombinant multi-epitope chimeric proteins as diagnosis antigens, produced fair 
      agreement in the case of A/C-2 (0.20&lt;kappa&lt;0.40) and good agreement
      (0.60&lt;kappa&lt;0.80) when A/C-7.1 was used as capture antigen. Chimera A/C-4 were
      excluded from further study due to loss of reactivity with OspA-specific
      antibodies. CONCLUSIONS: The combination of specific B-cell epitopes from OspA
      and OspC proteins may improve the diagnostic accuracy of serologic assays, but
      further studies are required to address this hypothesis.
FAU - Schreterova, Eva
AU  - Schreterova E
AD  - Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology 
      and Immunology, University of Veterinary Medicine and Pharmacy, 041 81 Kosice,
      Slovakia. [email protected]
FAU - Bhide, Mangesh
AU  - Bhide M
AD  - Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology 
      and Immunology, University of Veterinary Medicine and Pharmacy, 041 81 Kosice,
      Slovakia. [email protected]
FAU - Potocnakova, Lenka
AU  - Potocnakova L
AD  - Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology 
      and Immunology, University of Veterinary Medicine and Pharmacy, 041 81 Kosice,
      Slovakia. [email protected]
FAU - Borszekova Pulzova, Lucia
AU  - Borszekova Pulzova L
AD  - Laboratory of Biomedical Microbiology and Immunology, Department of Microbiology 
      and Immunology, University of Veterinary Medicine and Pharmacy, 041 81 Kosice,
      Slovakia. [email protected]
LA  - eng
PT  - Evaluation Studies
PT  - Journal Article
DEP - 20171218
PL  - Poland
TA  - Ann Agric Environ Med
JT  - Annals of agricultural and environmental medicine : AAEM
JID - 9500166
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Surface)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Bacterial Vaccines)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (Lipoproteins)
RN  - 0 (OspA protein)
SB  - IM
MH  - Antibodies, Bacterial/analysis/immunology
MH  - Antigens, Surface/analysis/genetics/immunology
MH  - Bacterial Outer Membrane Proteins/analysis/genetics/immunology
MH  - Bacterial Vaccines/analysis/genetics/immunology
MH  - Borrelia burgdorferi/genetics/immunology/*isolation &amp; purification
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Epitopes, B-Lymphocyte/*analysis/immunology
MH  - Humans
MH  - Lipoproteins/analysis/genetics/immunology
MH  - Lyme Disease/*diagnosis/immunology/microbiology
OTO - NOTNLM
OT  - ELISA
OT  - Lyme disease
OT  - Slovakia
OT  - chimera
OT  - epitope
EDAT- 2017/12/30 06:00
MHDA- 2018/01/06 06:00
CRDT- 2017/12/30 06:00
PHST- 2017/12/30 06:00 [entrez]
PHST- 2017/12/30 06:00 [pubmed]
PHST- 2018/01/06 06:00 [medline]
AID - 80699 [pii]
AID - 10.26444/aaem/80699 [doi]
PST - ppublish
SO  - Ann Agric Environ Med. 2017 Dec 23;24(4):696-701. doi: 10.26444/aaem/80699. Epub 
      2017 Dec 18.