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Effect of Different Disinfection/Sterilization Methods on Risk of Fracture of Teeth Used in Preclinical Dental Education.

Abstract The aim of this in vitro study was to determine whether different disinfection/sterilization methods affected the risk of fracture of extracted teeth used for preclinical dental education. Freshly extracted intact mandibular incisors were assigned to different groups according to the processing method used. In the autoclave group (n=20), teeth were autoclaved for 40 min at 240°F under a pressure of 20 psi; in the formalin group (n=20), teeth were immersed in 10% formalin for two weeks; and in the control group (n=10), teeth were not processed. Teeth were then stored at 4°C in distilled water until use. Endodontic procedures were performed, and the fracture strength of the specimen was subsequently tested under compressive force along the long axis of the teeth using an Instron universal testing machine. The results showed that none of the specimens fractured during endodontic procedures. However, the compressive load needed to fracture the teeth was significantly less for the autoclaved teeth than the teeth stored in formalin or the control teeth (p<0.001). The disinfection/sterilization method used affected the fracture resistance of extracted teeth: autoclaved teeth were less resistant to fracture than teeth that were not sterilized or teeth that were chemically disinfected. However, fracture resistance was not reduced enough to lead to tooth fracture during preclinical endodontic procedures. Therefore, either processing method may be appropriate for teeth to be used for preclinical endodontic training.
PMID
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Authors

Mayor MeshTerms

Education, Dental

Keywords

dental education

endodontics

extracted teeth

fracture strength

sterilization

Journal Title journal of dental education
Publication Year Start




PMID- 29292330
OWN - NLM
STAT- MEDLINE
DCOM- 20180112
LR  - 20180112
IS  - 1930-7837 (Electronic)
IS  - 0022-0337 (Linking)
VI  - 82
IP  - 1
DP  - 2018 Jan
TI  - Effect of Different Disinfection/Sterilization Methods on Risk of Fracture of
      Teeth Used in Preclinical Dental Education.
PG  - 84-87
LID - 10.21815/JDE.018.012 [doi]
AB  - The aim of this in vitro study was to determine whether different
      disinfection/sterilization methods affected the risk of fracture of extracted
      teeth used for preclinical dental education. Freshly extracted intact mandibular 
      incisors were assigned to different groups according to the processing method
      used. In the autoclave group (n=20), teeth were autoclaved for 40 min at 240
      degrees F under a pressure of 20 psi; in the formalin group (n=20), teeth were
      immersed in 10% formalin for two weeks; and in the control group (n=10), teeth
      were not processed. Teeth were then stored at 4 degrees C in distilled water
      until use. Endodontic procedures were performed, and the fracture strength of the
      specimen was subsequently tested under compressive force along the long axis of
      the teeth using an Instron universal testing machine. The results showed that
      none of the specimens fractured during endodontic procedures. However, the
      compressive load needed to fracture the teeth was significantly less for the
      autoclaved teeth than the teeth stored in formalin or the control teeth
      (p&lt;0.001). The disinfection/sterilization method used affected the fracture
      resistance of extracted teeth: autoclaved teeth were less resistant to fracture
      than teeth that were not sterilized or teeth that were chemically disinfected.
      However, fracture resistance was not reduced enough to lead to tooth fracture
      during preclinical endodontic procedures. Therefore, either processing method may
      be appropriate for teeth to be used for preclinical endodontic training.
FAU - Michaud, Pierre-Luc
AU  - Michaud PL
AD  - Dr. Michaud is Assistant Professor, Department of Dental Clinical Sciences,
      Faculty of Dentistry, Dalhousie University; Mahdis Maleki is a dental student,
      Faculty of Dentistry, Dalhousie University; and Dr. Mello is Associate Professor 
      and Division Head of Endodontics, Department of Dental Clinical Sciences, Faculty
      of Dentistry, Dalhousie University.
FAU - Maleki, Mahdis
AU  - Maleki M
AD  - Dr. Michaud is Assistant Professor, Department of Dental Clinical Sciences,
      Faculty of Dentistry, Dalhousie University; Mahdis Maleki is a dental student,
      Faculty of Dentistry, Dalhousie University; and Dr. Mello is Associate Professor 
      and Division Head of Endodontics, Department of Dental Clinical Sciences, Faculty
      of Dentistry, Dalhousie University.
FAU - Mello, Isabel
AU  - Mello I
AD  - Dr. Michaud is Assistant Professor, Department of Dental Clinical Sciences,
      Faculty of Dentistry, Dalhousie University; Mahdis Maleki is a dental student,
      Faculty of Dentistry, Dalhousie University; and Dr. Mello is Associate Professor 
      and Division Head of Endodontics, Department of Dental Clinical Sciences, Faculty
      of Dentistry, Dalhousie University. [email protected]
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Dent Educ
JT  - Journal of dental education
JID - 8000150
SB  - D
SB  - IM
MH  - Disinfection
MH  - *Education, Dental
MH  - Humans
MH  - In Vitro Techniques
MH  - Risk Assessment
MH  - Sterilization/*methods
MH  - Tooth Extraction
MH  - Tooth Fractures/*epidemiology/etiology
OTO - NOTNLM
OT  - dental education
OT  - endodontics
OT  - extracted teeth
OT  - fracture strength
OT  - sterilization
EDAT- 2018/01/03 06:00
MHDA- 2018/01/13 06:00
CRDT- 2018/01/03 06:00
PHST- 2017/06/12 00:00 [received]
PHST- 2017/07/30 00:00 [accepted]
PHST- 2018/01/03 06:00 [entrez]
PHST- 2018/01/03 06:00 [pubmed]
PHST- 2018/01/13 06:00 [medline]
AID - 82/1/84 [pii]
AID - 10.21815/JDE.018.012 [doi]
PST - ppublish
SO  - J Dent Educ. 2018 Jan;82(1):84-87. doi: 10.21815/JDE.018.012.