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MiR-34a regulates the invasive capacity of canine osteosarcoma cell lines.

Abstract Osteosarcoma (OSA) is the most common bone tumor in children and dogs; however, no substantial improvement in clinical outcome has occurred in either species over the past 30 years. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and play a fundamental role in cancer. The purpose of this study was to investigate the potential contribution of miR-34a loss to the biology of canine OSA, a well-established spontaneous model of the human disease.
PMID
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Journal Title plos one
Publication Year Start




PMID- 29293555
OWN - NLM
STAT- In-Data-Review
LR  - 20180102
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 13
IP  - 1
DP  - 2018
TI  - MiR-34a regulates the invasive capacity of canine osteosarcoma cell lines.
PG  - e0190086
LID - 10.1371/journal.pone.0190086 [doi]
AB  - BACKGROUND: Osteosarcoma (OSA) is the most common bone tumor in children and
      dogs; however, no substantial improvement in clinical outcome has occurred in
      either species over the past 30 years. MicroRNAs (miRNAs) are small non-coding
      RNAs that regulate gene expression and play a fundamental role in cancer. The
      purpose of this study was to investigate the potential contribution of miR-34a
      loss to the biology of canine OSA, a well-established spontaneous model of the
      human disease. METHODOLOGY AND PRINCIPAL FINDINGS: RT-qPCR demonstrated that
      miR-34a expression levels were significantly reduced in primary canine OSA tumors
      and canine OSA cell lines as compared to normal canine osteoblasts. In canine OSA
      cell lines stably transduced with empty vector or pre-miR-34a lentiviral
      constructs, overexpression of miR-34a inhibited cellular invasion and migration
      but had no effect on cell proliferation or cell cycle distribution.
      Transcriptional profiling of canine OSA8 cells possessing enforced miR-34a
      expression demonstrated dysregulation of numerous genes, including significant
      down-regulation of multiple putative targets of miR-34a. Moreover, gene ontology 
      analysis of down-regulated miR-34a target genes showed enrichment of several
      biological processes related to cell invasion and motility. Lastly, we validated 
      changes in miR-34a putative target gene expression, including decreased
      expression of KLF4, SEM3A, and VEGFA transcripts in canine OSA cells
      overexpressing miR-34a and identified KLF4 and VEGFA as direct target genes of
      miR-34a. Concordant with these data, primary canine OSA tumor tissues
      demonstrated increased expression levels of putative miR-34a target genes.
      CONCLUSIONS: These data demonstrate that miR-34a contributes to invasion and
      migration in canine OSA cells and suggest that loss of miR-34a may promote a
      pattern of gene expression contributing to the metastatic phenotype in canine
      OSA.
FAU - Lopez, Cecilia M
AU  - Lopez CM
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The
      Ohio State University, Columbus, Ohio, United States of America.
FAU - Yu, Peter Y
AU  - Yu PY
AD  - Medical Student Research Program, The Ohio State University College of Medicine, 
      The Ohio State University Wexner Medical Center, Columbus, Ohio, United States of
      America.
FAU - Zhang, Xiaoli
AU  - Zhang X
AD  - Center for Biostatistics, Department of Biomedical Informatics, The Ohio State
      University, Columbus, Ohio, United States of America.
FAU - Yilmaz, Ayse Selen
AU  - Yilmaz AS
AD  - Center for Biostatistics, Department of Biomedical Informatics, The Ohio State
      University, Columbus, Ohio, United States of America.
FAU - London, Cheryl A
AU  - London CA
AD  - Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio
      State University, Columbus, Ohio, United States of America.
AD  - Department of Veterinary Biosciences, College of Veterinary Medicine, Tufts
      University, New Grafton, Massachusetts, United States of America.
FAU - Fenger, Joelle M
AU  - Fenger JM
AUID- ORCID: http://orcid.org/0000-0003-1407-8856
AD  - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The
      Ohio State University, Columbus, Ohio, United States of America.
LA  - eng
PT  - Journal Article
DEP - 20180102
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
EDAT- 2018/01/03 06:00
MHDA- 2018/01/03 06:00
CRDT- 2018/01/03 06:00
PHST- 2017/03/28 00:00 [received]
PHST- 2017/12/07 00:00 [accepted]
PHST- 2018/01/03 06:00 [entrez]
PHST- 2018/01/03 06:00 [pubmed]
PHST- 2018/01/03 06:00 [medline]
AID - 10.1371/journal.pone.0190086 [doi]
AID - PONE-D-17-07439 [pii]
PST - epublish
SO  - PLoS One. 2018 Jan 2;13(1):e0190086. doi: 10.1371/journal.pone.0190086.
      eCollection 2018.