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Biochemical characterisation of a Kunitz-type inhibitor from Tamarindus indica L. seeds and its efficacy in reducing plasma leptin in an experimental model of obesity.

Abstract A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, two protein fractions were observed: Fr1 and Fr2, with average mass of [M + 14H]+ = 19,594,690 Da and [M + 13H]+ = 19,578,266 Da, respectively. The protein fractions showed 54 and 53 amino acid residues with the same sequence. pTTI presented resistance to temperature and pH variations; IC50 was 2.7 × 10-10 mol.L-1 and Ki was 2.9 × 10-11 mol.L-1. The 2-DE revealed spots with isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with possible biotechnological health-related application.
PMID
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Authors

Mayor MeshTerms

Disease Models, Animal

Keywords

CCK

Tamarind

Wistar rats

antitryptic

Journal Title journal of enzyme inhibition and medicinal chemistry
Publication Year Start




PMID- 29322840
OWN - NLM
STAT- MEDLINE
DCOM- 20180115
LR  - 20180115
IS  - 1475-6374 (Electronic)
IS  - 1475-6366 (Linking)
VI  - 33
IP  - 1
DP  - 2018 Dec
TI  - Biochemical characterisation of a Kunitz-type inhibitor from Tamarindus indica L.
      seeds and its efficacy in reducing plasma leptin in an experimental model of
      obesity.
PG  - 334-348
LID - 10.1080/14756366.2017.1419220 [doi]
AB  - A trypsin inhibitor isolated from tamarind seed (TTI) has satietogenic effects in
      animals, increasing the cholecystokinin (CCK) in eutrophy and reducing leptin in 
      obesity. We purified TTI (pTTI), characterised, and observed its effect upon CCK 
      and leptin in obese Wistar rats. By HPLC, and after amplification of resolution, 
      two protein fractions were observed: Fr1 and Fr2, with average mass of [M +
      14H](+) = 19,594,690 Da and [M + 13H](+) = 19,578,266 Da, respectively. The
      protein fractions showed 54 and 53 amino acid residues with the same sequence.
      pTTI presented resistance to temperature and pH variations; IC50 was 2.7 x 10(-10
      )mol.L(-1) and Ki was 2.9 x 10(-11 )mol.L(-1). The 2-DE revealed spots with
      isoelectric points between pH 5 and 6, and one near pH 8. pTTI action on leptin
      decrease was confirmed. We conclude that pTTI is a Kunitz trypsin inhibitor with 
      possible biotechnological health-related application.
FAU - Medeiros, Amanda Fernandes de
AU  - Medeiros AF
AD  - a Postgraduate Biochemistry Program, Biosciences Center , Federal University of
      Rio Grande do Norte , Natal , Brazil.
FAU - Costa, Izael de Sousa
AU  - Costa IS
AD  - a Postgraduate Biochemistry Program, Biosciences Center , Federal University of
      Rio Grande do Norte , Natal , Brazil.
FAU - Carvalho, Fabiana Maria Coimbra de
AU  - Carvalho FMC
AD  - a Postgraduate Biochemistry Program, Biosciences Center , Federal University of
      Rio Grande do Norte , Natal , Brazil.
FAU - Kiyota, Sumika
AU  - Kiyota S
AD  - b Laboratory of Protein and Peptide Biochemistry , CPDSA, Biological Institute , 
      Sao Paulo , Brazil.
FAU - Souza, Beatriz Blenda Pinheiro de
AU  - Souza BBP
AD  - c Postgraduate Biological Molecular, Institute of Biological Sciences ,
      University of Brasilia , Distrito Federal , Brasilia , Brazil.
AD  - d Embrapa Genetic Resources and Biotechnology , Embrapa , Distrito Federal ,
      Brasilia , Brazil.
FAU - Sifuentes, Daniel Nogoceke
AU  - Sifuentes DN
AD  - d Embrapa Genetic Resources and Biotechnology , Embrapa , Distrito Federal ,
      Brasilia , Brazil.
FAU - Serquiz, Raphael Paschoal
AU  - Serquiz RP
AD  - a Postgraduate Biochemistry Program, Biosciences Center , Federal University of
      Rio Grande do Norte , Natal , Brazil.
FAU - Maciel, Bruna Leal Lima
AU  - Maciel BLL
AD  - e Postgraduate Nutrition Program, Center for Health Sciences , Federal University
      of Rio Grande do Norte , Natal , Brazil.
AD  - f Department of Nutrition, Center for Health Sciences , Federal University of Rio
      Grande do Norte , Natal , Brazil.
FAU - Uchoa, Adriana Ferreira
AU  - Uchoa AF
AD  - a Postgraduate Biochemistry Program, Biosciences Center , Federal University of
      Rio Grande do Norte , Natal , Brazil.
AD  - g Department of Cell Biology and Genetics, Biosciences Center , Federal
      University of Rio Grande do Norte , Natal , Brazil.
FAU - Santos, Elizeu Antunes Dos
AU  - Santos EAD
AD  - a Postgraduate Biochemistry Program, Biosciences Center , Federal University of
      Rio Grande do Norte , Natal , Brazil.
AD  - h Department of Biochemistry, Biosciences Center , Federal University of Rio
      Grande do Norte , Natal , Brazil.
FAU - Morais, Ana Heloneida de Araujo
AU  - Morais AHA
AD  - a Postgraduate Biochemistry Program, Biosciences Center , Federal University of
      Rio Grande do Norte , Natal , Brazil.
AD  - e Postgraduate Nutrition Program, Center for Health Sciences , Federal University
      of Rio Grande do Norte , Natal , Brazil.
AD  - f Department of Nutrition, Center for Health Sciences , Federal University of Rio
      Grande do Norte , Natal , Brazil.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Enzyme Inhib Med Chem
JT  - Journal of enzyme inhibition and medicinal chemistry
JID - 101150203
RN  - 0 (Anti-Obesity Agents)
RN  - 0 (Kunitz-type protease inhibitor, plant)
RN  - 0 (Leptin)
RN  - 0 (Peptides)
RN  - 0 (Plant Proteins)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Animals
MH  - Anti-Obesity Agents/chemistry/isolation & purification/*pharmacology
MH  - *Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Leptin/*blood
MH  - Male
MH  - Obesity/*blood/*drug therapy/metabolism
MH  - Peptides/chemistry/isolation & purification/*pharmacology
MH  - Plant Proteins/chemistry/isolation & purification/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Seeds/chemistry
MH  - Structure-Activity Relationship
MH  - Tamarindus/*chemistry
MH  - Trypsin/metabolism
OTO - NOTNLM
OT  - CCK
OT  - Tamarind
OT  - Wistar rats
OT  - antitryptic
EDAT- 2018/01/13 06:00
MHDA- 2018/01/16 06:00
CRDT- 2018/01/12 06:00
PHST- 2018/01/12 06:00 [entrez]
PHST- 2018/01/13 06:00 [pubmed]
PHST- 2018/01/16 06:00 [medline]
AID - 10.1080/14756366.2017.1419220 [doi]
PST - ppublish
SO  - J Enzyme Inhib Med Chem. 2018 Dec;33(1):334-348. doi:
      10.1080/14756366.2017.1419220.