PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Diversity analysis of subgingival microbial bacteria in peri-implantitis in Uygur population.

Abstract This study is to investigate the subgingival bacterial diversity and community structure in the Uygur subjects with peri-implantitis.Totally 40 cases of gingival crevicular fluid were collected from Uygur subjects and divided into the Control group (healthy implants) and Case group (peri-implantitis), respectively. DNA was extracted, and the sequencing in the 16SrRNA V4-V5 region was conducted on the Illumina Miseq sequencing platform. The 16SrRNA gene clone library was constructed and analyzed.Totally 733,759 valid tags were obtained from these 40 samples. After comparing with the Silva-16S database by the Uparse software, 263 operational taxonomic unit were finally harvested (135 for the Control group and 128 for the Case group). The differential bacteria between these 2 groups at the phylum, class, order, family, and genus levels were Actinobacteria, Actinomycetes, Pasteurellales, Moraxellaceae, and Acinetobacter, respectively. The dominant genera with significantly different distribution between the Control and Case groups included Vibrio, Campylobacter, Granulicatella, Acinetobacter, Micrococcus, and Moraxella. The α diverstiy analysis based on the chao diversity index showed that there was significant difference in the microbiological diversity between these 2 groups. Principal coordinates analysis analysis indicated significant differences in the bacterial community structure between these 2 groups. Cluster analysis showed higher abundance of Micrococcus in the Case group, while higher abundance of Prevotella in the Control group.There are significant differences in the diversity of subgingival bacteria between the Uygur subjects with healthy implants and peri-implantitis. Moraxella, Micrococcus, and Acinetobacter might represent dominant bacteria genera causing peri-implantitis in the Uygur population.
PMID
Related Publications

Clinical, microbiological, and immunological aspects of healthy versus peri-implantitis tissue in full arch reconstruction patients: a prospective cross-sectional study.

Protein biomarkers and microbial profiles in peri-implantitis.

Subgingival microbiome in patients with healthy and ailing dental implants.

Microbiological diversity of peri-implantitis biofilm by Sanger sequencing.

Study on Microbial Diversity of Peri-implantitis Subgingival by High-throughput Sequencing.

Authors

Mayor MeshTerms
Keywords
Journal Title medicine
Publication Year Start




PMID- 29384870
OWN - NLM
STAT- MEDLINE
DCOM- 20180209
LR  - 20180221
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Linking)
VI  - 97
IP  - 5
DP  - 2018 Feb
TI  - Diversity analysis of subgingival microbial bacteria in peri-implantitis in Uygur
      population.
PG  - e9774
LID - 10.1097/MD.0000000000009774 [doi]
AB  - This study is to investigate the subgingival bacterial diversity and community
      structure in the Uygur subjects with peri-implantitis.Totally 40 cases of
      gingival crevicular fluid were collected from Uygur subjects and divided into the
      Control group (healthy implants) and Case group (peri-implantitis), respectively.
      DNA was extracted, and the sequencing in the 16SrRNA V4-V5 region was conducted
      on the Illumina Miseq sequencing platform. The 16SrRNA gene clone library was
      constructed and analyzed.Totally 733,759 valid tags were obtained from these 40
      samples. After comparing with the Silva-16S database by the Uparse software, 263 
      operational taxonomic unit were finally harvested (135 for the Control group and 
      128 for the Case group). The differential bacteria between these 2 groups at the 
      phylum, class, order, family, and genus levels were Actinobacteria,
      Actinomycetes, Pasteurellales, Moraxellaceae, and Acinetobacter, respectively.
      The dominant genera with significantly different distribution between the Control
      and Case groups included Vibrio, Campylobacter, Granulicatella, Acinetobacter,
      Micrococcus, and Moraxella. The alpha diverstiy analysis based on the chao
      diversity index showed that there was significant difference in the
      microbiological diversity between these 2 groups. Principal coordinates analysis 
      analysis indicated significant differences in the bacterial community structure
      between these 2 groups. Cluster analysis showed higher abundance of Micrococcus
      in the Case group, while higher abundance of Prevotella in the Control
      group.There are significant differences in the diversity of subgingival bacteria 
      between the Uygur subjects with healthy implants and peri-implantitis. Moraxella,
      Micrococcus, and Acinetobacter might represent dominant bacteria genera causing
      peri-implantitis in the Uygur population.
FAU - Gao, Xiaowei
AU  - Gao X
AD  - Department of Implantation, School of Stomatology, Jilin University, Changchun,
      Jilin, China.
FAU - Zhou, Jing
AU  - Zhou J
AD  - School of Stomatology, Lanzhou University, Lanzhou, Gansu, China.
FAU - Sun, Xiaolin
AU  - Sun X
AD  - Department of Implantation, School of Stomatology, Jilin University, Changchun,
      Jilin, China.
FAU - Li, Xue
AU  - Li X
AD  - Department of Implantation, School of Stomatology, Jilin University, Changchun,
      Jilin, China.
FAU - Zhou, Yanmin
AU  - Zhou Y
AD  - Department of Implantation, School of Stomatology, Jilin University, Changchun,
      Jilin, China.
LA  - eng
PT  - Journal Article
PT  - Observational Study
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
SB  - AIM
SB  - IM
MH  - Adult
MH  - Bacteria/classification/*isolation & purification
MH  - Bacterial Typing Techniques
MH  - Case-Control Studies
MH  - China
MH  - Female
MH  - Gingival Crevicular Fluid/*microbiology
MH  - Humans
MH  - Male
MH  - Microbiota
MH  - Middle Aged
MH  - Peri-Implantitis/*microbiology
PMC - PMC5805442
EDAT- 2018/02/01 06:00
MHDA- 2018/02/10 06:00
CRDT- 2018/02/01 06:00
PHST- 2018/02/01 06:00 [entrez]
PHST- 2018/02/01 06:00 [pubmed]
PHST- 2018/02/10 06:00 [medline]
AID - 10.1097/MD.0000000000009774 [doi]
AID - 00005792-201802020-00032 [pii]
PST - ppublish
SO  - Medicine (Baltimore). 2018 Feb;97(5):e9774. doi: 10.1097/MD.0000000000009774.