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Functional analysis of cell-free RNA using mid-trimester amniotic fluid supernatant in pregnancy with the fetal growth restriction.

Abstract The prediction and monitoring of fetal growth restriction (FGR) fetuses has become with the use of ultrasound. However, these tools lack the fundamental evidence for the growth of fetus with FGR excluding pathogenic factors.Amniotic fluid samples were obtained from pregnant women for fetal karyotyping and genetic diagnosis at 16 to 19 weeks of gestation. For this study, 15 FGR and 9 control samples were selected, and cell-free fetal RNA was isolated from each supernatant of the amniotic fluid for microarray analysis.In this study, 411 genes were differentially expressed between the FGR and control group. Of these genes, 316 genes were up-regulated, while 95 genes were down-regulated. In terms of gene ontology, the up-regulated genes were highly related to metabolic process as well as protein synthesis, while the down-regulated genes were related to receptor activity and biological adhesion. In terms of tissue-specific expression, the up-regulated genes were involved in various organs while down-regulated genes were involved only in the brain. In terms of organ-specific expression, many genes were enriched for B-cell lymphoma, pancreas, eye, placenta, epithelium, skin, and muscle. In the functional significance of gene, low-density lipoprotein receptor-related protein 10 (LRP10) was significantly increased (6-fold) and insulin-like growth factor (IGF-2) was dramatically increased (17-fold) in the FGR cases.The results show that the important brain-related genes are predominantly down-regulated in the intrauterine growth restriction fetuses during the second trimester of pregnancy. This study also suggested possible genes related to fetal development such as B-cell lymphoma, LRP10, and IGF-2. To monitor the fetal development, further study may be needed to elucidate the role of the genes identified.
PMID
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Authors

Mayor MeshTerms
Keywords
Journal Title medicine
Publication Year Start




PMID- 29480850
OWN - NLM
STAT- MEDLINE
DCOM- 20180305
LR  - 20180305
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Linking)
VI  - 97
IP  - 2
DP  - 2018 Jan
TI  - Functional analysis of cell-free RNA using mid-trimester amniotic fluid
      supernatant in pregnancy with the fetal growth restriction.
PG  - e9572
LID - 10.1097/MD.0000000000009572 [doi]
AB  - The prediction and monitoring of fetal growth restriction (FGR) fetuses has
      become with the use of ultrasound. However, these tools lack the fundamental
      evidence for the growth of fetus with FGR excluding pathogenic factors.Amniotic
      fluid samples were obtained from pregnant women for fetal karyotyping and genetic
      diagnosis at 16 to 19 weeks of gestation. For this study, 15 FGR and 9 control
      samples were selected, and cell-free fetal RNA was isolated from each supernatant
      of the amniotic fluid for microarray analysis.In this study, 411 genes were
      differentially expressed between the FGR and control group. Of these genes, 316
      genes were up-regulated, while 95 genes were down-regulated. In terms of gene
      ontology, the up-regulated genes were highly related to metabolic process as well
      as protein synthesis, while the down-regulated genes were related to receptor
      activity and biological adhesion. In terms of tissue-specific expression, the
      up-regulated genes were involved in various organs while down-regulated genes
      were involved only in the brain. In terms of organ-specific expression, many
      genes were enriched for B-cell lymphoma, pancreas, eye, placenta, epithelium,
      skin, and muscle. In the functional significance of gene, low-density lipoprotein
      receptor-related protein 10 (LRP10) was significantly increased (6-fold) and
      insulin-like growth factor (IGF-2) was dramatically increased (17-fold) in the
      FGR cases.The results show that the important brain-related genes are
      predominantly down-regulated in the intrauterine growth restriction fetuses
      during the second trimester of pregnancy. This study also suggested possible
      genes related to fetal development such as B-cell lymphoma, LRP10, and IGF-2. To 
      monitor the fetal development, further study may be needed to elucidate the role 
      of the genes identified.
CI  - Copyright (c) 2017 The Authors. Published by Wolters Kluwer Health, Inc. All
      rights reserved.
FAU - Cho, Hee Young
AU  - Cho HY
AD  - Department of Obstetrics and Gynecology, CHA Bundang Medical Center, CHA
      University, Seongnam.
FAU - Cho, Yeonkyung
AU  - Cho Y
AD  - Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA
      University.
FAU - Shin, Yun-Jeong
AU  - Shin YJ
AD  - Genetic Laboratory, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
FAU - Park, Jieun
AU  - Park J
AD  - Genetic Laboratory, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
FAU - Shim, Sunghan
AU  - Shim S
AD  - Genetic Laboratory, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
FAU - Jung, Yongwook
AU  - Jung Y
AD  - Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA
      University.
FAU - Shim, Sungshin
AU  - Shim S
AD  - Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA
      University.
FAU - Cha, Donghyun
AU  - Cha D
AD  - Department of Obstetrics and Gynecology, CHA Gangnam Medical Center, CHA
      University.
AD  - Genetic Laboratory, CHA Gangnam Medical Center, CHA University, Seoul, Korea.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 63231-63-0 (RNA)
SB  - AIM
SB  - IM
MH  - Adult
MH  - Amniotic Fluid/*chemistry/metabolism
MH  - Female
MH  - Fetal Growth Retardation/*diagnosis/metabolism
MH  - Humans
MH  - Male
MH  - Microarray Analysis
MH  - Pregnancy
MH  - Pregnancy Trimester, Second
MH  - Prenatal Care
MH  - Prospective Studies
MH  - RNA/*analysis/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Transcriptome
EDAT- 2018/02/27 06:00
MHDA- 2018/03/06 06:00
CRDT- 2018/02/27 06:00
PHST- 2018/02/27 06:00 [entrez]
PHST- 2018/02/27 06:00 [pubmed]
PHST- 2018/03/06 06:00 [medline]
AID - 10.1097/MD.0000000000009572 [doi]
AID - 00005792-201801120-00030 [pii]
PST - ppublish
SO  - Medicine (Baltimore). 2018 Jan;97(2):e9572. doi: 10.1097/MD.0000000000009572.