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Expression of cytokines in aqueous humor from fungal keratitis patients.

Abstract Although a series of reports on corneal fungal infection have been published, studies on pathogenic mechanisms and inflammation-associated cytokines remain limited. In this study, aqueous humor samples from fungal keratitis patients were collected to examine cytokine patterns and cellular profile for the pathogenesis of fungal keratitis.
PMID
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Authors

Mayor MeshTerms
Keywords

aqueous humor

cytokines

fungal keratitis

inflammation

Journal Title bmc ophthalmology
Publication Year Start




PMID- 29673332
OWN - NLM
STAT- MEDLINE
DCOM- 20180430
LR  - 20180501
IS  - 1471-2415 (Electronic)
IS  - 1471-2415 (Linking)
VI  - 18
IP  - 1
DP  - 2018 Apr 19
TI  - Expression of cytokines in aqueous humor from fungal keratitis patients.
PG  - 105
LID - 10.1186/s12886-018-0754-x [doi]
AB  - BACKGROUND: Although a series of reports on corneal fungal infection have been
      published, studies on pathogenic mechanisms and inflammation-associated cytokines
      remain limited. In this study, aqueous humor samples from fungal keratitis
      patients were collected to examine cytokine patterns and cellular profile for the
      pathogenesis of fungal keratitis. METHODS: The aqueous humor samples were
      collected from ten patients with advanced stage fungal keratitis. Eight aqueous
      humor samples from patients with keratoconus or corneal dystrophy were taken as
      control. Approximately 100 mul to 300 mul of aqueous humor in each case were
      obtained for examination. The aqueous humor samples were centrifuged and the
      cells were stained and examined under optical microscope. Bacterial and fungal
      cultures were performed on the aqueous humor and corneal buttons of all patients.
      Cytokines related to inflammation including IL-1beta, IL-6, IL-8, IL-10,
      TNF-alpha, and IFN-gamma were examined using multiplex bead-based Luminex liquid 
      protein array systems. RESULTS: Fungus infection was confirmed in these ten
      patients by smear stains and/or fungal cultures. Bacterial and fungal cultures
      revealed negative results in all aqueous humor specimens. Polymorphonuclear
      leukocytes were the predominant infiltrating cells in the aqueous humor of fungal
      keratitis. At the advanced stages of fungal keratitis, the levels of IL-1beta,
      IL-6, IL-8, and IFN-gamma in the aqueous humor were significantly increased when 
      compared with control (p<0.01). The levels of IL-10 and TNF-alpha also showed an 
      ascending trend but with no statistical significance. CONCLUSIONS: High
      concentration of IL-1beta, IL-6, IL-8, and IFN-gamma in the aqueous humor was
      associated with fungal keratitis.
FAU - Zhang, Yingnan
AU  - Zhang Y
AD  - Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University,
      Beijing Ophthalmology & Visual Science Key Lab, Beijing, 100730, China.
FAU - Liang, Qingfeng
AU  - Liang Q
AD  - Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren
      Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and
      Visual Sciences, Beijing, 100005, China.
FAU - Liu, Yang
AU  - Liu Y
AD  - Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University,
      Beijing Ophthalmology & Visual Science Key Lab, Beijing, 100730, China.
FAU - Pan, Zhiqiang
AU  - Pan Z
AD  - Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University,
      Beijing Ophthalmology & Visual Science Key Lab, Beijing, 100730, China.
      [email protected]
FAU - Baudouin, Christophe
AU  - Baudouin C
AD  - Quinze-Vingts National Ophthalmology Hospital, Paris and Versailles
      Saint-Quentin-en-Yvelines University, Versailles, France.
AD  - INSERM, U968, Paris, F-75012, France; UPMC Univ Paris 06, UMR_S 968, Institut de 
      la Vision, Paris F-75012, France; CNRS, UMR_7210, Paris F-75012, France, Paris,
      France.
FAU - Labbe, Antoine
AU  - Labbe A
AD  - Quinze-Vingts National Ophthalmology Hospital, Paris and Versailles
      Saint-Quentin-en-Yvelines University, Versailles, France.
AD  - INSERM, U968, Paris, F-75012, France; UPMC Univ Paris 06, UMR_S 968, Institut de 
      la Vision, Paris F-75012, France; CNRS, UMR_7210, Paris F-75012, France, Paris,
      France.
FAU - Lu, Qingxian
AU  - Lu Q
AD  - Department of Ophthalmology and Visual Sciences, University of Louisville, 301 E.
      Muhammad Ali Blvd, Louisville, KY, 40202, USA.
LA  - eng
GR  - No.81541105/National Natural Scientific Foundation of China
PT  - Journal Article
DEP - 20180419
PL  - England
TA  - BMC Ophthalmol
JT  - BMC ophthalmology
JID - 100967802
RN  - 0 (Cytokines)
RN  - 0 (Interleukins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aqueous Humor/*metabolism
MH  - Case-Control Studies
MH  - Cytokines/*metabolism
MH  - Eye Infections, Fungal/*metabolism/microbiology
MH  - Female
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukins/metabolism
MH  - Keratitis/*metabolism/microbiology
MH  - Male
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Young Adult
PMC - PMC5907746
OTO - NOTNLM
OT  - aqueous humor
OT  - cytokines
OT  - fungal keratitis
OT  - inflammation
EDAT- 2018/04/21 06:00
MHDA- 2018/05/01 06:00
CRDT- 2018/04/21 06:00
PHST- 2017/08/22 00:00 [received]
PHST- 2018/03/29 00:00 [accepted]
PHST- 2018/04/21 06:00 [entrez]
PHST- 2018/04/21 06:00 [pubmed]
PHST- 2018/05/01 06:00 [medline]
AID - 10.1186/s12886-018-0754-x [doi]
AID - 10.1186/s12886-018-0754-x [pii]
PST - epublish
SO  - BMC Ophthalmol. 2018 Apr 19;18(1):105. doi: 10.1186/s12886-018-0754-x.