PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 29690696
OWN - NLM
STAT- MEDLINE
DCOM- 20180501
LR  - 20180501
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 53
IP  - 4
DP  - 2018 Apr 9
TI  - [Expression and potential clinical significance of cytidine triphosphate:
      phosphocholine cytidylyltransferase-alpha in oral squamous cell carcinoma].
PG  - 254-258
LID - 10.3760/cma.j.issn.1002-0098.2018.04.008 [doi]
AB  - Objective: To examine the expression and potential clinical significance of CCT
      (cytidine triphosphate: phosphocholine cytidylyltransferase)-alpha in oral
      squamous cell carcinoma (OSCC). Methods: Fifty-eight OSCC and paired adjacent
      non-malignant epithelia samples (between May 2016 and July 2016) were obtained
      from dental center, Second Xiangya Hospital, Central South University. CCT-alpha 
      expression was examined by immunohistochemistry. The relationship between
      CCT-alpha and clinicopathological features of OSCC patients was analyzed.
      Quantitative real-time PCR and Western blot were performed to measure the
      expression of CCT-alpha mRNA and protein level in several OSCC cell line and two 
      normal oral epithelial cell line. Results: Immunohistochemistry showed that
      CCT-alpha positive staining was found in cell nuclear of OSCC cells and adjacent 
      epithelial cells. CCT-alpha was positively expressed in OSCC, which was
      significantly higher than that adjacent to carcinoma tissues (P=0.000). The
      expression of CCT-alpha in oral squamous cell carcinoma was correlated with
      smoking, alcohol consumption, tumor size, differentiation degree and lymph node
      metastasis. The expression level of CCT-alpha protein was significantly increased
      in patients with a history of smoking and alcohol consumption (P=0.001, P=0.004).
      With the increase of tumor diameter, the expression of CCT-alpha protein was
      significantly increased (P=0.005). According to histopathological grade, the
      lower the degree of tumor differentiation, the higher the expression level of
      CCT-alpha protein (P=0.000). The expression of CCT-alpha protein was
      significantly higher in patients with lymph node metastasis compared with no
      lymph node metastasis (P=0.000). Quantitative real-time PCR results showed the
      CCT-alpha mRNA expression level was significantly higher in OSCC cells than that 
      in normal oral epithelial cells (P=0.016). The protein expression level of
      CCT-alpha was significantly higher in OSCC cells than that in normal oral
      epithelial cells. Conclusions: CCT-alpha may play a critical role in the
      carcinogenesis and development of OSCC.
FAU - Lu, F C
AU  - Lu FC
AD  - Dental Center, Second Xiangya Hospital, Central South University, Changsha
      410011, China.
FAU - Xie, X Y
AU  - Xie XY
AD  - Dental Center, Second Xiangya Hospital, Central South University, Changsha
      410011, China.
FAU - Yin, X M
AU  - Yin XM
AD  - Department of Stomatology, Xiangya Hospital, Central South University, Changsha
      410008, China.
FAU - Gao, Y J
AU  - Gao YJ
AD  - Dental Center, Second Xiangya Hospital, Central South University, Changsha
      410011, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese
      journal of stomatology
JID - 8711066
RN  - 0 (RNA, Messenger)
RN  - 65-47-4 (Cytidine Triphosphate)
RN  - EC 2.7.7.15 (Choline-Phosphate Cytidylyltransferase)
SB  - D
SB  - IM
MH  - Blotting, Western
MH  - Carcinoma, Squamous Cell/*metabolism
MH  - Choline-Phosphate Cytidylyltransferase/*metabolism
MH  - Cytidine Triphosphate/*metabolism
MH  - Epithelial Cells/metabolism
MH  - Epithelium/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Lymphatic Metastasis
MH  - Mouth Neoplasms/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Cytidine triphosphate: phosphocholine cytidylyltransferase-alpha
OT  - Mouth neoplasms
OT  - Oral sprays
EDAT- 2018/04/25 06:00
MHDA- 2018/05/02 06:00
CRDT- 2018/04/26 06:00
PHST- 2018/04/26 06:00 [entrez]
PHST- 2018/04/25 06:00 [pubmed]
PHST- 2018/05/02 06:00 [medline]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2018 Apr 9;53(4):254-258.