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IL-10 polymorphisms and T-cell subsets could affect the clinical presentation and outcome of childhood immune thrombocytopenia in Egyptian population.

Abstract The aim is to study IL-10 polymorphisms and IL-10 level and assess their relation to T-cell subsets in childhood immune thrombocytopenia (ITP). In all, 40 (25 acute, 15 chronic) ITP child patients were investigated at time of presentation, compared to 15 healthy, age- and gender-matched controls and followed up for 1 year to determine chronic cases. Studying the effect of IL-10 promoter polymorphism was done by PCR-RFLP, IL-10 level was determined by ELISA, natural killer cells and T-cell subsets were evaluated by flow cytometry. Subjects with IL-10 promoter (1082 AA and 592 AA) genotypes had lower IL-10 levels and had lower CD4%, higher CD8%, lower CD4/CD8 ratio and lower T-reg%. IL-10 polymorphisms had no effect on NK%. IL-10 serum levels and IL-10 promoter polymorphic genotype frequencies are not different between ITP cases and controls; however, in ITP patients, IL-10 promoter (1082 AA and 592 AA) genotypes and associated lower CD4, higher CD8, lower CD4/CD8 ratio is associated with more severe thrombocytopenia at presentation and had a poorer response to first-line treatment. Patients with lower T-reg cells had a higher tendency to develop chronic ITP. IL-10 level and polymorphisms as well as disturbed T-cell subsets percentages are demonstrable effectors of immune dysfunction in ITP and can affect the presentation and outcome of childhood ITP.
PMID
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Authors

Mayor MeshTerms

Polymorphism, Genetic

Keywords

IL-10

Idiopathic thrombocytopenic purpura

T cells

Journal Title apmis : acta pathologica, microbiologica, et immunologica scandinavica
Publication Year Start




PMID- 29696721
OWN - NLM
STAT- MEDLINE
DCOM- 20180503
LR  - 20180503
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 126
IP  - 5
DP  - 2018 May
TI  - IL-10 polymorphisms and T-cell subsets could affect the clinical presentation and
      outcome of childhood immune thrombocytopenia in Egyptian population.
PG  - 380-388
LID - 10.1111/apm.12823 [doi]
AB  - The aim is to study IL-10 polymorphisms and IL-10 level and assess their relation
      to T-cell subsets in childhood immune thrombocytopenia (ITP). In all, 40 (25
      acute, 15 chronic) ITP child patients were investigated at time of presentation, 
      compared to 15 healthy, age- and gender-matched controls and followed up for 1
      year to determine chronic cases. Studying the effect of IL-10 promoter
      polymorphism was done by PCR-RFLP, IL-10 level was determined by ELISA, natural
      killer cells and T-cell subsets were evaluated by flow cytometry. Subjects with
      IL-10 promoter (1082 AA and 592 AA) genotypes had lower IL-10 levels and had
      lower CD4%, higher CD8%, lower CD4/CD8 ratio and lower T-reg%. IL-10
      polymorphisms had no effect on NK%. IL-10 serum levels and IL-10 promoter
      polymorphic genotype frequencies are not different between ITP cases and
      controls; however, in ITP patients, IL-10 promoter (1082 AA and 592 AA) genotypes
      and associated lower CD4, higher CD8, lower CD4/CD8 ratio is associated with more
      severe thrombocytopenia at presentation and had a poorer response to first-line
      treatment. Patients with lower T-reg cells had a higher tendency to develop
      chronic ITP. IL-10 level and polymorphisms as well as disturbed T-cell subsets
      percentages are demonstrable effectors of immune dysfunction in ITP and can
      affect the presentation and outcome of childhood ITP.
CI  - (c) 2018 APMIS. Published by John Wiley & Sons Ltd.
FAU - Soliman, Mohamed A
AU  - Soliman MA
AD  - Department of Clinical Pathology, Faculty of Medicine, Menoufia University,
      Menoufia, Egypt.
FAU - Helwa, Mohamed A
AU  - Helwa MA
AD  - Department of Clinical Pathology, Faculty of Medicine, Menoufia University,
      Menoufia, Egypt.
FAU - Fath-Allah, Samar K
AU  - Fath-Allah SK
AD  - Department of Clinical Pathology, Faculty of Medicine, Menoufia University,
      Menoufia, Egypt.
FAU - El-Hawy, Mahmoud A
AU  - El-Hawy MA
AD  - Department of Pediatrics, Faculty of Medicine, Menoufia University, Menoufia,
      Egypt.
FAU - Badr, Hassan S
AU  - Badr HS
AD  - Department of Pediatrics, Faculty of Medicine, Menoufia University, Menoufia,
      Egypt.
FAU - Barseem, Naglaa Fathy
AU  - Barseem NF
AD  - Department of Pediatrics, Faculty of Medicine, Menoufia University, Menoufia,
      Egypt.
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (IL10 protein, human)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - CD4-CD8 Ratio
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Infant
MH  - Interleukin-10/*genetics
MH  - Male
MH  - *Polymorphism, Genetic
MH  - Promoter Regions, Genetic
MH  - Purpura, Thrombocytopenic, Idiopathic/genetics/*immunology
MH  - T-Lymphocyte Subsets/*immunology
OTO - NOTNLM
OT  - IL-10
OT  - Idiopathic thrombocytopenic purpura
OT  - T cells
EDAT- 2018/04/27 06:00
MHDA- 2018/05/04 06:00
CRDT- 2018/04/27 06:00
PHST- 2017/09/23 00:00 [received]
PHST- 2018/01/26 00:00 [accepted]
PHST- 2018/04/27 06:00 [entrez]
PHST- 2018/04/27 06:00 [pubmed]
PHST- 2018/05/04 06:00 [medline]
AID - 10.1111/apm.12823 [doi]
PST - ppublish
SO  - APMIS. 2018 May;126(5):380-388. doi: 10.1111/apm.12823.