PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.




PMID- 29715103
OWN - NLM
STAT- MEDLINE
DCOM- 20180515
LR  - 20180515
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 38
IP  - 5
DP  - 2018 May
TI  - Bidirectional Regulation of COX-2 Expression Between Cancer Cells and
      Macrophages.
PG  - 2811-2817
AB  - BACKGROUND/AIM: Our aim was to investigate the crosstalk between tumor and immune
      cells (M2 macrophages) and its effects on cyclo-oxygenase-2 (COX2) regulation in 
      canine mammary tumors (CMT). MATERIALS AND METHODS: Sh1b CMT cells and human
      BT474 mammary or HT29 colon cancer cells were co-cultured with canine peripheral 
      blood mononuclear cells (PBMCs) or with macrophage-like differentiated THP1
      monocytes (dTHP1). Intracellular COX2 expression by PBMCs, dTHP1 and cancer cells
      was evaluated by flow cytometry. RESULTS: Co-culturing of Sh1b and canine PBMCs
      induced COX2 overexpression in CMT cells. In turn, COX2 expression by PBMCs,
      mostly CD68(+) macrophages, was attenuated by co-culture with Sh1b (p=0.0001). In
      accordance, co-culture with dTHP1 prompted intracellular production of COX2 in
      both Sh1b CMT cells and HT29 human colon cancer cells and reduced production of
      COX2 in BT474 human mammary cancer cells. The intracellular COX2 expression from 
      dTHP1 decreased when treated with conditioned medium from cultured Sh1b and HT29 
      cancer cells. CONCLUSION: Bidirectional COX2 regulation between cancer and
      monocytes/macrophages might shape a tolerogenic tumor microenvironment in CMT.
CI  - Copyright(c) 2018, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Carvalho, Maria Isabel
AU  - Carvalho MI
AD  - Animal and Veterinary Research Centre, University of Tras-os-Montes and Alto
      Douro, Vila Real, Portugal.
AD  - Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro,
      Vila Real, Portugal.
AD  - The Interuniversity Messerli Research Institute of the University of Veterinary
      Medicine Vienna, Medical University Vienna and University Vienna, Vienna,
      Austria.
FAU - Bianchini, Rodolfo
AU  - Bianchini R
AD  - The Interuniversity Messerli Research Institute of the University of Veterinary
      Medicine Vienna, Medical University Vienna and University Vienna, Vienna,
      Austria.
FAU - Fazekas-Singer, Judit
AU  - Fazekas-Singer J
AD  - The Interuniversity Messerli Research Institute of the University of Veterinary
      Medicine Vienna, Medical University Vienna and University Vienna, Vienna,
      Austria.
AD  - Institute of Pathophysiology and Allergy Research, Center of Pathophysiology,
      Infectiology and Immunology, Medical University Vienna, Vienna, Austria.
FAU - Herrmann, Ina
AU  - Herrmann I
AD  - Division of Dermatology, Internal Medicine Small Animals, University Clinics of
      Horses and Small Animals, University of Veterinary Medicine, Vienna, Austria.
FAU - Flickinger, Irene
AU  - Flickinger I
AD  - Division of Oncology, Small Animal Internal Medicine, University Clinics of
      Horses and Small Animals, University of Veterinary Medicine, Vienna, Austria.
FAU - Thalhammer, Johann G
AU  - Thalhammer JG
AD  - Division of Dermatology, Internal Medicine Small Animals, University Clinics of
      Horses and Small Animals, University of Veterinary Medicine, Vienna, Austria.
FAU - Pires, Isabel
AU  - Pires I
AD  - Animal and Veterinary Research Centre, University of Tras-os-Montes and Alto
      Douro, Vila Real, Portugal.
AD  - Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro,
      Vila Real, Portugal.
FAU - Jensen-Jarolim, Erika
AU  - Jensen-Jarolim E
AD  - The Interuniversity Messerli Research Institute of the University of Veterinary
      Medicine Vienna, Medical University Vienna and University Vienna, Vienna,
      Austria.
AD  - Institute of Pathophysiology and Allergy Research, Center of Pathophysiology,
      Infectiology and Immunology, Medical University Vienna, Vienna, Austria.
FAU - Queiroga, Felisbina L
AU  - Queiroga FL
AD  - Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro,
      Vila Real, Portugal [email protected]
AD  - Center for the Study of Animal Sciences, Institute of Sciences, Technologies and 
      Agro-environment, University of Porto, Porto, Portugal.
AD  - Center for Research and Technology of Agro-Environment and Biological Sciences,
      University of Tras-os-Montes and Alto Douro, Vila Real, Portugal.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Cyclooxygenase 2/*biosynthesis
MH  - Dogs
MH  - Female
MH  - Humans
MH  - Macrophages/*metabolism
MH  - Mammary Neoplasms, Animal/immunology/*metabolism/pathology
MH  - Receptor Cross-Talk
MH  - Tumor Escape/*immunology
MH  - Tumor Microenvironment/*immunology
OTO - NOTNLM
OT  - *BT474
OT  - *COX-2
OT  - *Canine mammary cancer
OT  - *HT29
OT  - *Sh1b
OT  - *macrophages
EDAT- 2018/05/02 06:00
MHDA- 2018/05/16 06:00
CRDT- 2018/05/02 06:00
PHST- 2018/02/03 00:00 [received]
PHST- 2018/03/12 00:00 [revised]
PHST- 2018/03/14 00:00 [accepted]
PHST- 2018/05/02 06:00 [entrez]
PHST- 2018/05/02 06:00 [pubmed]
PHST- 2018/05/16 06:00 [medline]
AID - 38/5/2811 [pii]
AID - 10.21873/anticanres.12525 [doi]
PST - ppublish
SO  - Anticancer Res. 2018 May;38(5):2811-2817. doi: 10.21873/anticanres.12525.