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Long non-coding RNA taurine-upregulated gene 1 predicts unfavorable prognosis, promotes cells proliferation, and inhibits cells apoptosis in epithelial ovarian cancer.

Abstract The aim of this study was to evaluate the correlation of long non-coding RNAs (lncRNAs) taurine-upregulated gene 1 (TUG1) with clinicopathological characteristics as well as overall survival (OS) in epithelial ovarian cancer (EOC) patients, and investigate its function in EOC cells proliferation and apoptosis in vitro.LncRNA TUG1 expressions were detected in tumor tissues and paired adjacent tissues obtained from 96 EOC patients. Blank mimic, lncRNA TUG1 mimic, blank inhibitor, and lncRNA TUG1 inhibitor plasmids were transfected into SKOV3 cells. CKK-8, annexin V-FITC-propidium iodide, qPCR and western blot assays were performed to detect cells proliferation, cells apoptosis, RNA expression, and protein expression, respectively.LncRNA TUG1 expression was higher in tumor tissue compared to paired adjacent tissue (P < .001), and it was positively correlated with pathological grade (P = .022), tumor size (P = .011) and FIGO stage (P < .001). Kaplan-Meier curve showed that lncRNA TUG1 high expression was associated with worse OS (P = .003). Multivariate Cox analysis indicated that lncRNA TUG1 high expression (vs. low expression) (P = .035) was independently predictive factor for shorter OS. In vitro, cells proliferation was promoted after treatment with lncRNA TUG1 mimic and was suppressed after treatment with lncRNA TUG1 inhibitor. In addition, cells apoptosis rate was decreased in lncRNA TUG1 mimic group compared to NC1 mimic, and increased in lncRNA TUG1 inhibitor group compared to NC2 inhibitor.In conclusion, lncRNA TUG1 is positively correlated with advanced disease and poor prognosis, and it promotes cells proliferation and inhibits cells apoptosis in EOC cells.
PMID
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Authors

Mayor MeshTerms

Neoplasms, Glandular and Epithelial

Ovarian Neoplasms

Keywords
Journal Title medicine
Publication Year Start




PMID- 29742691
OWN - NLM
STAT- MEDLINE
DCOM- 20180515
LR  - 20180515
IS  - 1536-5964 (Electronic)
IS  - 0025-7974 (Linking)
VI  - 97
IP  - 19
DP  - 2018 May
TI  - Long non-coding RNA taurine-upregulated gene 1 predicts unfavorable prognosis,
      promotes cells proliferation, and inhibits cells apoptosis in epithelial ovarian 
      cancer.
PG  - e0575
LID - 10.1097/MD.0000000000010575 [doi]
AB  - The aim of this study was to evaluate the correlation of long non-coding RNAs
      (lncRNAs) taurine-upregulated gene 1 (TUG1) with clinicopathological
      characteristics as well as overall survival (OS) in epithelial ovarian cancer
      (EOC) patients, and investigate its function in EOC cells proliferation and
      apoptosis in vitro.LncRNA TUG1 expressions were detected in tumor tissues and
      paired adjacent tissues obtained from 96 EOC patients. Blank mimic, lncRNA TUG1
      mimic, blank inhibitor, and lncRNA TUG1 inhibitor plasmids were transfected into 
      SKOV3 cells. CKK-8, annexin V-FITC-propidium iodide, qPCR and western blot assays
      were performed to detect cells proliferation, cells apoptosis, RNA expression,
      and protein expression, respectively.LncRNA TUG1 expression was higher in tumor
      tissue compared to paired adjacent tissue (P &lt; .001), and it was positively
      correlated with pathological grade (P = .022), tumor size (P = .011) and FIGO
      stage (P &lt; .001). Kaplan-Meier curve showed that lncRNA TUG1 high expression was 
      associated with worse OS (P = .003). Multivariate Cox analysis indicated that
      lncRNA TUG1 high expression (vs. low expression) (P = .035) was independently
      predictive factor for shorter OS. In vitro, cells proliferation was promoted
      after treatment with lncRNA TUG1 mimic and was suppressed after treatment with
      lncRNA TUG1 inhibitor. In addition, cells apoptosis rate was decreased in lncRNA 
      TUG1 mimic group compared to NC1 mimic, and increased in lncRNA TUG1 inhibitor
      group compared to NC2 inhibitor.In conclusion, lncRNA TUG1 is positively
      correlated with advanced disease and poor prognosis, and it promotes cells
      proliferation and inhibits cells apoptosis in EOC cells.
FAU - Li, Tong-Huai
AU  - Li TH
AD  - Department of Gynaecology &amp; Obstetrics.
FAU - Zhang, Jing-Jing
AU  - Zhang JJ
AD  - Department of Imaging, People's Hospital of Lishui City, the Sixth Affiliated
      Hospital of Wenzhou Medical University, Lishui, China.
FAU - Liu, Shao-Xiao
AU  - Liu SX
AD  - Department of Gynaecology &amp; Obstetrics.
FAU - Chen, Yan
AU  - Chen Y
AD  - Department of Gynaecology &amp; Obstetrics.
LA  - eng
PT  - Journal Article
PT  - Observational Study
PL  - United States
TA  - Medicine (Baltimore)
JT  - Medicine
JID - 2985248R
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TUG1 long noncoding RNA, human)
RN  - Ovarian epithelial cancer
SB  - AIM
SB  - IM
MH  - Apoptosis/physiology
MH  - Cell Proliferation/physiology
MH  - China
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - *Neoplasms, Glandular and Epithelial/genetics/metabolism/mortality/pathology
MH  - *Ovarian Neoplasms/genetics/metabolism/mortality/pathology
MH  - Prognosis
MH  - RNA, Long Noncoding/*genetics
EDAT- 2018/05/10 06:00
MHDA- 2018/05/16 06:00
CRDT- 2018/05/10 06:00
PHST- 2018/05/10 06:00 [entrez]
PHST- 2018/05/10 06:00 [pubmed]
PHST- 2018/05/16 06:00 [medline]
AID - 10.1097/MD.0000000000010575 [doi]
AID - 00005792-201805110-00010 [pii]
PST - ppublish
SO  - Medicine (Baltimore). 2018 May;97(19):e0575. doi: 10.1097/MD.0000000000010575.