PubTransformer

A site to transform Pubmed publications into these bibliographic reference formats: ADS, BibTeX, EndNote, ISI used by the Web of Knowledge, RIS, MEDLINE, Microsoft's Word 2007 XML.

Immunomodulation of peripheral T cells in chickens infected with Marek's disease virus: involvement in immunosuppression.

Abstract Marek's disease virus (MDV) causes T cell immunosuppression in chickens during latent infection. Morphological changes specific to apoptosis were demonstrated in peripheral blood mononuclear cells (PBMC) of MDV-infected chickens at 2-3 weeks post-inoculation (p.i.). Analysis of DNA fragmentation in T cell subsets in the peripheral blood revealed that CD4+ T cells but not CD8+ T cells underwent apoptosis after MDV infection. The proportion of CD4+ T cells, but not that of CD8+ T cells, in the peripheral blood expanded transiently at 16 days p.i., and rapidly decreased 1 week later. The decrease in CD4+ T cells might be mediated by apoptosis, because a rapid reduction in CD4+ T cells was observed when these cells underwent apoptosis. Analysis of the T cell-receptor (TCR) repertoire of the peripheral blood showed that V beta 1 but not V beta 2-alpha beta TCR-bearing cells expanded at 16 days p.i., when the transient expansion of the CD4+ T cell population was observed in these chickens. Flow cytometric profiles also showed that the expression of CD8 was down-regulated after infection with MDV, but there was no difference in the expression level of CD4 molecules between normal and infected chickens. Northern blot analysis indicated that the down-regulation of CD8 occurred at the transcriptional level. These results suggest that both apoptosis of CD4+ T cells and down-regulation of CD8 molecules could contribute to the immunosuppression caused by MDV.
PMID
Related Publications

Cytokine gene expression in splenic CD4(+) and CD8(+) T-cell subsets of chickens infected with Marek's disease virus.

Detection of the meq gene in the T cell subsets from chickens infected with Marek's disease virus serotype 1.

Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells.

Characterization of Marek's disease herpesvirus-specific cytotoxic T lymphocytes in chickens inoculated with a non-oncogenic vaccine strain of MDV.

Apoptosis in peripheral CD4+T cells and thymocytes by Marek's disease virus-infection.

Authors

Mayor MeshTerms

Immune Tolerance

Keywords
Journal Title the journal of general virology
Publication Year Start




PMID- 8847503
OWN - NLM
STAT- MEDLINE
DA  - 19961021
DCOM- 19961021
LR  - 20031114
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 76 ( Pt 12)
DP  - 1995 Dec
TI  - Immunomodulation of peripheral T cells in chickens infected with Marek's disease 
      virus: involvement in immunosuppression.
PG  - 2979-85
AB  - Marek's disease virus (MDV) causes T cell immunosuppression in chickens during
      latent infection. Morphological changes specific to apoptosis were demonstrated
      in peripheral blood mononuclear cells (PBMC) of MDV-infected chickens at 2-3
      weeks post-inoculation (p.i.). Analysis of DNA fragmentation in T cell subsets in
      the peripheral blood revealed that CD4+ T cells but not CD8+ T cells underwent
      apoptosis after MDV infection. The proportion of CD4+ T cells, but not that of
      CD8+ T cells, in the peripheral blood expanded transiently at 16 days p.i., and
      rapidly decreased 1 week later. The decrease in CD4+ T cells might be mediated by
      apoptosis, because a rapid reduction in CD4+ T cells was observed when these
      cells underwent apoptosis. Analysis of the T cell-receptor (TCR) repertoire of
      the peripheral blood showed that V beta 1 but not V beta 2-alpha beta TCR-bearing
      cells expanded at 16 days p.i., when the transient expansion of the CD4+ T cell
      population was observed in these chickens. Flow cytometric profiles also showed
      that the expression of CD8 was down-regulated after infection with MDV, but there
      was no difference in the expression level of CD4 molecules between normal and
      infected chickens. Northern blot analysis indicated that the down-regulation of
      CD8 occurred at the transcriptional level. These results suggest that both
      apoptosis of CD4+ T cells and down-regulation of CD8 molecules could contribute
      to the immunosuppression caused by MDV.
FAU - Morimura, T
AU  - Morimura T
AD  - Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido
      University, Sapporo, Japan.
FAU - Hattori, M
AU  - Hattori M
FAU - Ohashi, K
AU  - Ohashi K
FAU - Sugimoto, C
AU  - Sugimoto C
FAU - Onuma, M
AU  - Onuma M
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antigens, CD8)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
SB  - X
MH  - Animals
MH  - Antigens, CD8/analysis
MH  - Apoptosis
MH  - Base Sequence
MH  - CD4-Positive T-Lymphocytes/chemistry/cytology/*immunology
MH  - CD8-Positive T-Lymphocytes/chemistry/cytology/*immunology
MH  - Chickens/*immunology
MH  - Down-Regulation
MH  - Herpesvirus 2, Gallid/*immunology
MH  - *Immune Tolerance
MH  - Marek Disease/*immunology
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis
MH  - Receptors, Antigen, T-Cell, alpha-beta/analysis
MH  - T-Lymphocyte Subsets/chemistry/cytology/immunology
EDAT- 1995/12/01
MHDA- 1995/12/01 00:01
CRDT- 1995/12/01 00:00
AID - 10.1099/0022-1317-76-12-2979 [doi]
PST - ppublish
SO  - J Gen Virol. 1995 Dec;76 ( Pt 12):2979-85.

<?xml version="1.0" encoding="UTF-8"?>
<b:Sources SelectedStyle="" xmlns:b="http://schemas.openxmlformats.org/officeDocument/2006/bibliography"  xmlns="http://schemas.openxmlformats.org/officeDocument/2006/bibliography" >
</b:Sources>